Jian-Pi-Yi-Shen drug-containing serum promotes proliferation and activity of mouse osteoblasts

Jian-Pi-Yi-Shen (JPYS) decoction is a traditional Chinese herbal formula. The present study aimed to investigate the effect of JPYS drug-containing serum on proliferation and activity mouse osteoblasts. Sprague‒Dawley rats were fed decoction, calcium supplement, or normal saline for 6 weeks, was collected. Mouse osteoblasts treated with serum, supplement blank control serum. Cell assayed using thiazolyl blue tetrazolium bromide. Levels alkaline phosphatase, nitric oxide, oxide synthase in culture medium measured. significantly improved compared It also increased levels phosphatase Treatment 48 h 72 (NO) concentration NOS by All these results enhanced that promotes These effects may be underlying mechanisms treating osteoporosis.